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GoldenGate Software Inc
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Thermo Fisher
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Bio-Rad
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FUJIFILM
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Becton Dickinson
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Thermo Fisher
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Raptor Photonics
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Thermo Fisher
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Image Search Results
Journal: Scientific Reports
Article Title: Impaired myogenesis in limb girdle muscular dystrophy type 2B
doi: 10.1038/s41598-025-10205-9
Figure Lengend Snippet: Silencing of the DYSF gene by CRISPR/Cas9 successfully reduces the synthesis of the dysferlin protein in myotubes from the control cell line. ( A ) Immunofluorescence staining indicating the absence of DYSF in the edited cell line, and the presence of myosin, showing that the cells maintain their capacity to differentiate. ( B ) Protein detection by western blotting highlights the reduction of the dysferlin protein to the levels of the LGMD2B myotubes and shows a secondary reduction of the calpain-3 protein, a finding already observed in other LGMD2B patients. Internal WB control for protein content in each sample can be observed with labelling to dystrophin (DMD), dysferlin (DYSF) and calpain3 (CAPN3).
Article Snippet: Sanger sequencing was performed to identify possible mutations and estimate the efficiency of gene editing, and we analyzed the .abi resulting files in the online software
Techniques: CRISPR, Control, Immunofluorescence, Staining, Western Blot
Journal: PLoS Genetics
Article Title: Early Lineage Priming by Trisomy of Erg Leads to Myeloproliferation in a Down Syndrome Model
doi: 10.1371/journal.pgen.1005211
Figure Lengend Snippet: A. Differential expression of murine orthologs of HSA21 trisomic genes in Ts65 (17 16 )Dn hematopoietic cells . Heatmap diagram of log 2 fold expression changes for genes with detectable expression in microarray data from the LSK, CMP, GMP and MEP cells for comparison between Ts65DnErg +/+/+ and Erg +/+ genotypes. Trisomic genes with significantly upregulated gene expression in Ts65(17 16 )Dn cells using a false discovery rate of 5% are indicated in red with an asterix. B. Barcode plot demonstrating distribution of differentially expressed genes due specifically to trisomy of Erg (pairwise comparison Ts65Dn/Erg +/+/+ versus Ts65Dn/Erg +/+/Mld2 ; red bars: upregulated genes; blue bars: downregulated genes) relative to gene expression changes due to overall trisomy (pairwise comparison Ts65Dn/Erg +/+/+ versus Erg +/+ ) in LSK cells. C. Venn diagram of differentially regulated genes in LSK cells due to the effect of overall trisomy ( Ts65Dn/Erg +/+/+ versus Erg +/+ ), trisomy of Erg ( Ts65Dn/Erg +/+/+ versus Ts65Dn/Erg +/+/Mld2 ) and non-Erg trisomy (Ts65Dn/Erg +/+/Mld2 versus Erg +/+ ). Intersect of differentially expressed genes by t-stastistic using an FDR of < 5%; (red) intersect of differentially expressed genes due to trisomy and Erg trisomy; (yellow) non-intersecting differentially expressed genes due to trisomy; (blue) non intersecting differentially expressed genes due to trisomy of Erg . D. Dot plot of differentially expressed genes in LSK cells by t-statistic comparing expression differences resulting from overall trisomy ( Ts65Dn/Erg +/+/+ versus Erg +/+ ) to those specifically attributable to Erg trisomy ( Ts65Dn/Erg +/+/+ versus Ts65Dn/Erg +/+/Mld2 , left panel) and Erg trisomy compared to expression changes specific for non -Erg genes in trisomy (Ts65Dn/Erg +/+/Mld2 versus Erg +/+ , right panel). Genes identified in the Venn diagram (Fig 2C) are shown using the same color scheme suggesting a high degree of correlation between differentially expressed genes due to trisomy and Erg trisomy in LSK cells.
Article Snippet:
Techniques: Expressing, Microarray